Physiological and proteomic responses of two contrasting Populus cathayana populations to drought stress.
Identifieur interne : 003530 ( Main/Exploration ); précédent : 003529; suivant : 003531Physiological and proteomic responses of two contrasting Populus cathayana populations to drought stress.
Auteurs : Xiangwen Xiao [République populaire de Chine] ; Fan Yang ; Sheng Zhang ; Helena Korpelainen ; Chunyang LiSource :
- Physiologia plantarum [ 1399-3054 ] ; 2009.
Descripteurs français
- KwdFr :
- Acclimatation (MeSH), Eau (métabolisme), Espèces réactives de l'oxygène (métabolisme), Feuilles de plante (métabolisme), Malonaldéhyde (analyse), Photosynthèse (MeSH), Populus (génétique), Populus (métabolisme), Populus (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéome (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse MALDI (MeSH), Stress physiologique (MeSH), Sécheresses (MeSH).
- MESH :
- analyse : Malonaldéhyde.
- génétique : Populus, Protéines végétales.
- métabolisme : Eau, Espèces réactives de l'oxygène, Feuilles de plante, Populus, Protéines végétales, Protéome.
- physiologie : Populus.
- Acclimatation, Photosynthèse, Régulation de l'expression des gènes végétaux, Spectrométrie de masse MALDI, Stress physiologique, Sécheresses.
English descriptors
- KwdEn :
- Acclimatization (MeSH), Droughts (MeSH), Gene Expression Regulation, Plant (MeSH), Malondialdehyde (analysis), Photosynthesis (MeSH), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), Populus (physiology), Proteome (metabolism), Reactive Oxygen Species (metabolism), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Stress, Physiological (MeSH), Water (metabolism).
- MESH :
- chemical , analysis : Malondialdehyde.
- chemical , genetics : Plant Proteins.
- genetics : Populus.
- metabolism : Plant Leaves, Plant Proteins, Populus, Proteome, Reactive Oxygen Species, Water.
- physiology : Populus.
- Acclimatization, Droughts, Gene Expression Regulation, Plant, Photosynthesis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Stress, Physiological.
Abstract
The acclimation of plants to water deficit is the result of many different physiological and biochemical mechanisms. To gain a better understanding of drought stress acclimation and tolerance mechanisms in Populus cathayana Rehder, we carried out an integrated physiological and comparative proteomic analysis on the drought stress responses of two contrasting populations originating from wet and dry regions in western China. The plantlets were subjected to continuous drought stress by withholding soil water content at 25% of field capacity (FC) for 45 days, while the control treatments were kept at 100% FC. Drought stress significantly inhibited plant growth, decreased net photosynthetic rate and stomatal conductance of leaves, increased the relative electrolyte leakage and malondialdehyde (MDA) content, and, at the same time, accumulated soluble sugars and free proline in both populations tested. The population from the dry climate region exhibited stronger tolerance to drought stress compared with the wet climate population. The proteomic analyses resulted in the identification of 40 drought-responsive proteins. The functional categories of these proteins include the regulation of transcription and translation, photosynthesis, cytoskeleton, secondary metabolism, HSPs/chaperones, redox homeostasis and defense response. The results suggest that poplars' tolerance to drought stress relates to the control of reactive oxygen species (ROS) and to osmoprotective capacity. The differential regulation of some drought-responsive proteins, such as HSPs and the enzymes related to redox homeostasis and regulation of secondary metabolism, plays an important role in poplars' tolerance and acclimation to drought stress. In conclusion, acclimation to water deficit involves changes in cellular metabolism and the regulation of gene networks. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in different poplar populations but also provides clues for improving poplars' drought tolerance through breeding or genetic engineering.
DOI: 10.1111/j.1399-3054.2009.01222.x
PubMed: 19453505
Affiliations:
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sort="Li, Chunyang" uniqKey="Li C" first="Chunyang" last="Li">Chunyang Li</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acclimatization (MeSH)</term><term>Droughts (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Malondialdehyde (analysis)</term><term>Photosynthesis (MeSH)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (physiology)</term><term>Proteome (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Stress, Physiological (MeSH)</term><term>Water (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acclimatation (MeSH)</term><term>Eau (métabolisme)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Feuilles de plante (métabolisme)</term><term>Malonaldéhyde (analyse)</term><term>Photosynthèse (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Sécheresses (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Malondialdehyde</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Malonaldéhyde</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Plant Proteins</term><term>Populus</term><term>Proteome</term><term>Reactive Oxygen Species</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Eau</term><term>Espèces réactives de l'oxygène</term><term>Feuilles de plante</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acclimatization</term><term>Droughts</term><term>Gene Expression Regulation, Plant</term><term>Photosynthesis</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acclimatation</term><term>Photosynthèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Spectrométrie de masse MALDI</term><term>Stress physiologique</term><term>Sécheresses</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The acclimation of plants to water deficit is the result of many different physiological and biochemical mechanisms. To gain a better understanding of drought stress acclimation and tolerance mechanisms in Populus cathayana Rehder, we carried out an integrated physiological and comparative proteomic analysis on the drought stress responses of two contrasting populations originating from wet and dry regions in western China. The plantlets were subjected to continuous drought stress by withholding soil water content at 25% of field capacity (FC) for 45 days, while the control treatments were kept at 100% FC. Drought stress significantly inhibited plant growth, decreased net photosynthetic rate and stomatal conductance of leaves, increased the relative electrolyte leakage and malondialdehyde (MDA) content, and, at the same time, accumulated soluble sugars and free proline in both populations tested. The population from the dry climate region exhibited stronger tolerance to drought stress compared with the wet climate population. The proteomic analyses resulted in the identification of 40 drought-responsive proteins. The functional categories of these proteins include the regulation of transcription and translation, photosynthesis, cytoskeleton, secondary metabolism, HSPs/chaperones, redox homeostasis and defense response. The results suggest that poplars' tolerance to drought stress relates to the control of reactive oxygen species (ROS) and to osmoprotective capacity. The differential regulation of some drought-responsive proteins, such as HSPs and the enzymes related to redox homeostasis and regulation of secondary metabolism, plays an important role in poplars' tolerance and acclimation to drought stress. In conclusion, acclimation to water deficit involves changes in cellular metabolism and the regulation of gene networks. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in different poplar populations but also provides clues for improving poplars' drought tolerance through breeding or genetic engineering.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19453505</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>136</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Physiological and proteomic responses of two contrasting Populus cathayana populations to drought stress.</ArticleTitle><Pagination><MedlinePgn>150-68</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1399-3054.2009.01222.x</ELocationID><Abstract><AbstractText>The acclimation of plants to water deficit is the result of many different physiological and biochemical mechanisms. To gain a better understanding of drought stress acclimation and tolerance mechanisms in Populus cathayana Rehder, we carried out an integrated physiological and comparative proteomic analysis on the drought stress responses of two contrasting populations originating from wet and dry regions in western China. The plantlets were subjected to continuous drought stress by withholding soil water content at 25% of field capacity (FC) for 45 days, while the control treatments were kept at 100% FC. Drought stress significantly inhibited plant growth, decreased net photosynthetic rate and stomatal conductance of leaves, increased the relative electrolyte leakage and malondialdehyde (MDA) content, and, at the same time, accumulated soluble sugars and free proline in both populations tested. The population from the dry climate region exhibited stronger tolerance to drought stress compared with the wet climate population. The proteomic analyses resulted in the identification of 40 drought-responsive proteins. The functional categories of these proteins include the regulation of transcription and translation, photosynthesis, cytoskeleton, secondary metabolism, HSPs/chaperones, redox homeostasis and defense response. The results suggest that poplars' tolerance to drought stress relates to the control of reactive oxygen species (ROS) and to osmoprotective capacity. The differential regulation of some drought-responsive proteins, such as HSPs and the enzymes related to redox homeostasis and regulation of secondary metabolism, plays an important role in poplars' tolerance and acclimation to drought stress. In conclusion, acclimation to water deficit involves changes in cellular metabolism and the regulation of gene networks. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in different poplar populations but also provides clues for improving poplars' drought tolerance through breeding or genetic engineering.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Xiangwen</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Chengdu Institute of Biology, Chinese Academy of Sciences, P.O.Box 416, Chengdu 610041, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Fan</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Sheng</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Korpelainen</LastName><ForeName>Helena</ForeName><Initials>H</Initials></Author><Author 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PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19453505</ArticleId><ArticleId IdType="pii">PPL1222</ArticleId><ArticleId IdType="doi">10.1111/j.1399-3054.2009.01222.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Korpelainen, Helena" sort="Korpelainen, Helena" uniqKey="Korpelainen H" first="Helena" last="Korpelainen">Helena Korpelainen</name><name sortKey="Li, Chunyang" sort="Li, Chunyang" uniqKey="Li C" first="Chunyang" last="Li">Chunyang Li</name><name sortKey="Yang, Fan" sort="Yang, Fan" uniqKey="Yang F" first="Fan" last="Yang">Fan Yang</name><name sortKey="Zhang, Sheng" sort="Zhang, Sheng" uniqKey="Zhang S" first="Sheng" last="Zhang">Sheng Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Xiao, Xiangwen" sort="Xiao, Xiangwen" uniqKey="Xiao X" first="Xiangwen" last="Xiao">Xiangwen Xiao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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